Iron Assay Kit (BA0040)

Iron Assay Kit - Description

Assay Genie's iron assay kit is designed to measure total iron directly in serum without any pretreatment. The improved method utilizes a chromogen that forms a blue colored complex specifically with Fe²⁺. Fe³⁺ in the sample is reduced to Fe²⁺, thus allowing the assay for total iron concentration. The intensity of the color, measured at 590nm, is directly proportional to the iron concentration in the sample.

Applications

For quantitative determination of iron ions Fe³⁺ and/or Fe²⁺ and evaluation of drug effects on iron metabolism.

Iron Assay Kit - Key Features

• Sensitive and accurate. Linear detection range 27 ug/dL (4.8 ) to 1,000 ug/dL (179 ) iron in 96-well plate assay.
• Simple and high-throughput. The procedure involves addition of a single working reagent and incubation for 40 min. Can be readily automated as a high-throughput assay for thousands of samples per day.
• Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvette or 96-well plate assay.
• Low interference in biological samples. No pretreatments are needed. Assays can be directly performed on serum samples.

Iron Assay Kit - Data Sheet
Kit Includes	Reagent A: 50 mL Reagent B: 4 mL Reagent C: 4 mL Iron Standard: 1 mL 10 mg/dL Fe2+
Kit Requires	Pipeting devices and accessories.
Method of Detection	OD590nm
Detection Limit	27 ug/dL (4.8 )
Samples	Biological (e.g. serum) and environmental samples
Species	All
Protocol Length	30 min
Size	250 tests
Storage	Store Reagent A at room temperature and other reagents at 4 °C.
Shelf Life	12 months

More Details

Iron level in blood is a reliable diagnostic indicator of various disease states. Increased levels of iron concentration in blood are associated with blood loss, increased destruction of red blood cells (e.g. hemorrhage) or decreased blood cell survival, acute hepatitis, certain sideroachrestic anemias, ingestion of iron-rich diets, defects in iron storage (e.g. pernicious anemia). Decreased levels of blood iron may result from insufficient iron ingestion from diets, chronic blood loss pathologies, or increased demand on iron storage as during normal pregnancy. Simple, direct and automation-ready procedures for measuring iron concentrations find wide applications in research, drug discovery and environmental monitoring.