Immunology Recombinant Proteins
Human WWP2 Recombinant Protein (RPES1124)
- SKU:
- RPES1124
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Immunology
Description
Human WWP2 Recombinant Protein
WWP2 contains 1 C2 domain, 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain and 4 WW domains. It is an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. WWP2 can be detected in heart, throughout the brain, placenta, lung, liver, muscle, kidney and pancreas. It is also expressed in spleen and peripheral blood leukocytes. WWP2 polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. WWP2 ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. It also ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. WWP2 ubiquitinates RPB1 and promotes it to proteasomal degradation.
| Product Name: | Human WWP2 Recombinant Protein (RPES1124) |
| Product Code: | RPES1124 |
| Size: | 20µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | AIP2,WWp2-like |
| Accession: | O00308 |
| Sequence: | Met 1-Glu870 |
| Fusion tag: | N-His & GST |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human WWP2 (O00308) (Met1-Glu870) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus. |
| Purity: | > 90 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 126.7 kDa |
| AP Mol Mass: | 120 kDa |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% glycerol |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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