Human WHSC2 Recombinant Protein (RPPB5148)
- SKU:
- RPPB5148
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9H3P2
Description
| Product Name: | Human WHSC2 Recombinant Protein |
| Product Code: | RPPB5148 |
| Size: | 10µg |
| Species: | Human |
| Target: | WHSC2 |
| Synonyms: | Negative elongation factor A, NELF-A, Wolf-Hirschhorn syndrome candidate 2 protein, WHSC2, NELFA, FLJ10442, FLJ25112, P/OKcl.15. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The WHSC2 solution (0.25 mg/ml) 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MPGQRRALSP KMASMRESDT GLWLHNKLGA TDELWAPPSI ASLLTAAVID NIRLCFHGLS SAVKLKLLLG TLHLPRRTVD EMKGALMEII QLASLDSDPW VLMVADILKS FPDTGSLNLE LEEQNPNVQD ILGELREKVG ECEASAMLPL ECQYLNKNAL TTLAGPLTPP VKHFQLKRKP KSATLRAELL QKSTETAQQL KRSAGVPFHA KGRGLLRKMD TTTPLKGIPK QAPFRSPTAP SVFSPTGNRT PIPPSRTLLR KERGVKLLDI SELDMVGAGR EAKRRRKTLD AEVVEKPAKE ETVVENATPD YAAGLVSTQK LGSLNNEPAL PSTSYLPSTP SVVPASSYIP SSETPPAPSS REASRPPEEP SAPSPTLPAQ FKQRAPMYNS GLSPATPTPA APTSPLTPTT PPAVAPTTQT PPVAMVAPQT QAPAQQQPKK NLSLTREQMF AAQEMFKTAN KVTRPEKALI LGFMAGSREN PCQEQGDVIQ IKLSEHTEDL PKADGQGSTT MLVDTVFEMN YATGQWTRFK KYKPMTNVS |
WHSC2 (NELF-A) is a protein factor required for DRB-sensitive transcription. WHSC2 is one of the 5 components of the multisubunit NELF complex which cooperates with DSIF to repress RNA polymerase II elongation. The Wolf-Hirschhorn syndrome is a multiple malformation syndrome characterized by mental and developmental defects resulting from a hemizygous deletion of the distal short arm of chromosome 4 (4p16.3).
WHSC2 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 559 amino acids (1-539 a.a.) and having a molecular mass of 60.6kDa. The WHSC2 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | WHSC2: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. Probably required to interact with the RNA polymerase II complex. Belongs to the NELF-A family. 2 isoforms of the human protein are produced by alternative splicing.Protein type: Transcription, coactivator/corepressorChromosomal Location of Human Ortholog: 4p16.3Cellular Component: nucleoplasm; cytoplasmMolecular Function: protein binding; chromatin bindingBiological Process: transcription from RNA polymerase II promoter; viral reproduction; positive regulation of viral transcription; multicellular organismal development; RNA elongation from RNA polymerase II promoter; gene expression |
| UniProt Protein Details: | |
| NCBI Summary: | This gene is expressed ubiquitously with higher levels in fetal than in adult tissues. It encodes a protein sharing 93% sequence identity with the mouse protein. Wolf-Hirschhorn syndrome (WHS) is a malformation syndrome associated with a hemizygous deletion of the distal short arm of chromosome 4. This gene is mapped to the 165 kb WHS critical region, and may play a role in the phenotype of the WHS or Pitt-Rogers-Danks syndrome. The encoded protein is found to be capable of reacting with HLA-A2-restricted and tumor-specific cytotoxic T lymphocytes, suggesting a target for use in specific immunotherapy for a large number of cancer patients. This protein has also been shown to be a member of the NELF (negative elongation factor) protein complex that participates in the regulation of RNA polymerase II transcription elongation. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9H3P2 |
| NCBI GenInfo Identifier: | 212276499 |
| NCBI Gene ID: | 7469 |
| NCBI Accession: | Q9H3P2.3 |
| UniProt Secondary Accession: | Q9H3P2,O95392, A2A2T1 |
| UniProt Related Accession: | Q9H3P2 |
| Molecular Weight: | 57,277 Da |
| NCBI Full Name: | Negative elongation factor A |
| NCBI Synonym Full Names: | negative elongation factor complex member A |
| NCBI Official Symbol: | NELFA�� |
| NCBI Official Synonym Symbols: | WHSC2; NELF-A; P/OKcl.15�� |
| NCBI Protein Information: | negative elongation factor A; wolf-Hirschhorn syndrome candidate 2 protein |
| UniProt Protein Name: | Negative elongation factor A |
| UniProt Synonym Protein Names: | Wolf-Hirschhorn syndrome candidate 2 protein |
| Protein Family: | |
| UniProt Gene Name: | NELFA�� |
| UniProt Entry Name: | NELFA_HUMAN |
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