Human VAMP4 Recombinant Protein (RPPB5106)

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SKU:
RPPB5106
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O75379

Description

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Product Name:Human VAMP4 Recombinant Protein
Product Code:RPPB5106
Size:10µg
Species:Human
Target:VAMP4
Synonyms:Vesicle-associated membrane protein 4, VAMP-4, VAMP4, VAMP24.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The VAMP4 protein solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH

VAMP4 belongs to the synaptobrevin family who are involved in docking and/or fusion of vesicles with cell membrane. VAMP4 is enriched in the trans-Golgi network and may be a factor in the trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway which functions to eliminate an inhibitor (most likely synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Synaptobrevins, syntaxins, and SNAP25 are the chief components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane.

VAMP4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 123 amino acids (1-115 a.a.) and having a molecular mass of 14.5kDa.VAMP4 is fused to an 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:VAMP4: Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule. Belongs to the synaptobrevin family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Vesicle; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q24-q25

Cellular Component: SNARE complex; Golgi membrane; Golgi apparatus; cell surface; lysosome; integral to membrane; trans-Golgi network; endosome

Molecular Function:SNAP receptor activity; SNARE binding

Biological Process: regulation of Golgi to plasma membrane protein transport; ER to Golgi vesicle-mediated transport; exocytosis; Golgi to plasma membrane protein transport; microtubule cytoskeleton organization and biogenesis

NCBI Summary:Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. This protein may play a role in trans-Golgi network-to-endosome transport. [provided by RefSeq, Jul 2008]
UniProt Code:O75379
NCBI GenInfo Identifier:20141854
NCBI Gene ID:8674
NCBI Accession:O75379.2
UniProt Related Accession:O75379
Molecular Weight:Observed: 16KDPredicted: 17 kDa
NCBI Full Name:Vesicle-associated membrane protein 4
NCBI Synonym Full Names:vesicle associated membrane protein 4
NCBI Official Symbol:VAMP4��
NCBI Official Synonym Symbols:VAMP-4; VAMP24��
NCBI Protein Information:vesicle-associated membrane protein 4
UniProt Protein Name:Vesicle-associated membrane protein 4
Protein Family:Vesicle-associated membrane protein
UniProt Gene Name:VAMP4��
UniProt Entry Name:VAMP4_HUMAN
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