Signal Transduction Recombinant Proteins
Human UBE2M/UBC12 Recombinant Protein (RPES3853)
- SKU:
- RPES3853
Description
Human UBE2M/UBC12 Recombinant Protein
UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. In order to degrade a protein, the ubiquitin-editing complex targets it for degradation via at least three classes of enzymes: ubiquitin-activating enzymes; or E1s; ubiquitin-conjugating enzymes; or E2s; and ubiquitin-protein ligases; or E3s. It is known that the protein NEDD8, which can be conjugated to cellular proteins like Cdc53/culin, interacts with this. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1; however not RBX2; suggests that the RBX1-UBE2M complex neddylates particular target proteins, such as CUL1, CUL2, and CUL3. It is involved in cell proliferation and serves as a key cellular mechanism for the degradation of aberrant or short-lived proteins. Human UBE2M/UBC12 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
| Product Name: | Human UBE2M/UBC12 Recombinant Protein (RPES3853) |
| Product Code: | RPES3853 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBC12, UBE2M, |
| Accession: | P61081 |
| Sequence: | Met1-Lys183 |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human UBE2M is produced by our E.coli expression system and the target gene encoding Met1-Lys183 is expressed. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 20.9 kDa |
| AP Mol Mass: | 25 kDa |
| Formulation: | Supplied as a 0.2 µm filtered solution of 50mM HEPES;pH 7.5;2mM DTT;150mM NaCl;10% Glycerol. |
| Shipping: | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C. |
| Stability and Storage: | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Signal Transduction |
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