Twinfilin-1; also known as Protein A6; Protein tyrosine kinase 9; TWF1 and PTK9; is a cytoplasm protein which belongs to the actin-binding proteins ADF family and Twinfilin subfamily. Twinfilin-1 (TWF1 / PTK9 ) is a highly conserved actin monomer-binding protein that regulates cytoskeletal dynamics in organisms from yeast to mammals. In addition to the mammalian twinfilin-1; a second protein with approximately 65% sequence identity to twinfilin-1 exists in mouse and humans. TWF1 / PTK9 is expressed at high levels in the colon; testis; ovary; prostate and lung. It is expressed at lower levels in the brain; bladder and heart. It is not detected in liver. TWF1 / PTK9 is an actin-binding protein involved in motile and morphological processes. It inhibits actin polymerization; likely by sequestering G-actin. By capping the barbed ends of filaments; it also regulates motility. TWF1 / PTK9 seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles.
Product Name:
Human TWF1/Twinfilin Recombinant Protein (RPES0661)
Product Code:
RPES0661
Size:
20µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
A6,MGC23788,MGC41876,PTK9
Accession:
Q12792-4
Sequence:
Met 1-Asp 252
Fusion tag:
N-His & GST
Endotoxin:
Please contact us for more information.
Protein Construction:
A DNA sequence encoding the human TWF1 isoform 4 (Q12792-4) (Met 1-Asp 252) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Purity:
> 84 % as determined by reducing SDS-PAGE.
Mol Mass:
57 kDa
AP Mol Mass:
50 kDa
Formulation:
Lyophilized from sterile 50mM Tris; 500mM NaCl; pH 8.0
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
