Developmental Biology Recombinant Proteins
Human TrkB/NTRK2 Recombinant Protein (RPES0776)
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- SKU:
- RPES0776
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Developmental Biology
Description
system_update_altDatasheet
Human TrkB/NTRK2 Recombinant Protein
The TRK Family of Tyrosine Kinase Receptor consists of 3 members: TrkA, TrkB and TrkC. The three TRK family proteins have different ligand specificities. They connect to different neurotrophins, including NGF, BDNF, NT-3NT-4/5. TRKA binds NGF, TRKB binds BDNF and NT-3, TRKC binds NT-4/5. At the protein sequence level, human and rat TRKB have greater than 90% sequence identity and the proteins exbihit cross-species activity. TRKB is primarily expressed in the nervous system and it also expression in a wide variety of tissues with low levels.
| Product Name: | Human TrkB/NTRK2 Recombinant Protein (RPES0776) |
| Product Code: | RPES0776 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | Human Cells |
| Synonyms: | BDNF/NT-3 Growth Factors Receptor, GP145-TrkB, Trk-B, Neurotrophic Tyrosine Kinase Receptor Type 2, TrkB Tyrosine Kinase, Tropomyosin-Related Kinase B, NTRK2, TRKB |
| Accession: | Q16620 |
| Sequence: | Cys32-His430 |
| Fusion tag: | C-His |
| Activity: | Immobilized Human NT-3(Cat: PKSH032803) at 2µg/ml(100 ?l/well) can bind Human TrkB-His. The ED50 of Human TrkB-His is 3.777ug/ml . |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human TrkB is produced by our Mammalian expression system and the target gene encoding Cys32-His430 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 45.25 kDa |
| AP Mol Mass: | 60-105 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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