Human TNNI3 Recombinant Protein (RPPB5018)
- SKU:
- RPPB5018
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P19429
Description
| Product Name: | Human TNNI3 Recombinant Protein |
| Product Code: | RPPB5018 |
| Size: | 5µg |
| Species: | Human |
| Target: | TNNI3 |
| Synonyms: | Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless liquid formulation. |
| Formulation: | TNNI3 protein solution (0.25mg/ml) contains 30% glycerol, 20mM Tris-HCl buffer (pH 7.5),� 0.1M NaCl, 0.1mMPMSF & 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by both SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMADGSSD AAREPRPAPA PIRRRSSNYR AYATEPHAKK KSKISASRKL QLKTLLLQIA KQELEREAEERRGEKGRALS TRCQPLELAG LGFAELQDLC RQLHARVDKV DEERYDIEAK VTKNITEIAD LTQKIFDLRG KFKRPTLRRV RISADAMMQALLGARAKESL DLRAHLKQVK KEDTEKENRE VGDWRKNIDA LSGMEGRKKK FES |
Troponin I (TnI), troponin T (TnT) and troponin C (TnC) form the troponin complex of the thin filaments of striated muscle. TnI acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains 3 genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and isexpressed solely in cardiac muscle tissues. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM).
TNNI3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 233 amino acids (1-210 a.a) and having a molecular mass of 26.4 kDa.TNNI3 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family. |
| UniProt Protein Details: | Protein type:Actin-binding; Motility/polarity/chemotaxis; Motor Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: cytosol; sarcomere; troponin complex Molecular Function:actin binding; calcium channel inhibitor activity; calcium-dependent protein binding; metal ion binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; muscle filament sliding; negative regulation of ATPase activity; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Dilated, 2a; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Familial Restrictive, 1 |
| NCBI Summary: | Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008] |
| UniProt Code: | P19429 |
| NCBI GenInfo Identifier: | 136213 |
| NCBI Gene ID: | 7137 |
| NCBI Accession: | P19429.3 |
| UniProt Secondary Accession: | P19429,P48787, P23693, |
| UniProt Related Accession: | P19429 |
| Molecular Weight: | 24,008 Da |
| NCBI Full Name: | Troponin I, cardiac muscle |
| NCBI Synonym Full Names: | troponin I3, cardiac type |
| NCBI Official Symbol: | TNNI3�� |
| NCBI Official Synonym Symbols: | CMH7; RCM1; cTnI; CMD2A; TNNC1; CMD1FF�� |
| NCBI Protein Information: | troponin I, cardiac muscle |
| UniProt Protein Name: | Troponin I, cardiac muscle |
| UniProt Synonym Protein Names: | Cardiac troponin I |
| Protein Family: | Troponin |
| UniProt Gene Name: | TNNI3�� |
| UniProt Entry Name: | TNNI3_HUMAN |
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