Human Cell Biology ELISA Kits 5

Human TNF- alpha (Tumor Necrosis Factor Alpha) ELISA Kit (HUES01395)

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SKU:
HUES01395
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P01375
ELISA Type:
Sandwich
Synonyms:
DIF, TNF-alpha, TNFA, TNFSF2
Reactivity:
Human
Sample Type:
Serum, plasma and other biological fluids
Research Area:
Cell Biology
Assay Type:
Sandwich-ELISA
Sensitivity:
4.69 pg/mL
Range:
7.81-500 pg/mL
Recovery:
80%-120%
Frequently bought together:

Description

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Human TNF- alpha (Tumor Necrosis Factor Alpha) ELISA Kit

The Human TNF-alpha (Tumor Necrosis Factor-alpha) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of TNF-alpha levels in human samples including serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it an ideal tool for a wide range of research applications.TNF-alpha is a key cytokine involved in inflammation, immune response, and apoptosis. Dysregulation of TNF-alpha has been associated with various inflammatory diseases, autoimmune disorders, and cancer. By accurately measuring TNF-alpha levels, researchers can gain valuable insights into the pathogenesis of these diseases and develop potential therapeutic strategies.The Human TNF-alpha ELISA Kit from Assay Genie offers researchers a reliable method for detecting and quantifying TNF-alpha levels in human samples, providing a valuable tool for studying the role of TNF-alpha in disease pathophysiology and identifying potential therapeutic targets.

Product Name:Human TNF- alpha (Tumor Necrosis Factor Alpha) ELISA Kit
SKU:HUES01395
Size:96 Assays
Detection Method:Colorimetric method, ELISA, Sandwich
Assay type:Sandwich-ELISA
Assay time:3 h 30 min
Sensitivity:4.69 pg/mL
Detection range:7.81-500 pg/mL
Reovery:80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
ComponentSpecificationStorage
Micro ELISA Plate (Dismountable)96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T-20°C, 12 months
Reference Standard96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials-20°C, 12 months
Concentrated Biotinylated Detection Ab(100×)96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL-20°C, 12 months
Concentrated HRP Conjugate (100×)96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL-20°C (Protect from light), 12 months
Reference Standard & Sample Diluent96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL2–8°C, 12 months
Biotinylated Detection Ab Diluent96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL2–8°C, 12 months
HRP Conjugate Diluent96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL2–8°C, 12 months
Concentrated Wash Buffer(25×)96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL2–8°C, 12 months
Substrate Reagent96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL2–8°C (Protect from light)
Stop Solution96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL2–8°C
Plate Sealer96T/48T/24T: 5 pieces | 96T*5: 25 pieces2–8°C
Technical Manual1 copy-
Certificate of Analysis1 copy-
Linearity:
Serum (n=5)EDTA Plasma (n=5)Cell Culture Media (n=5)
1:2Range (%)92-10584-9788-99
Average (%)999193
1:4Range (%)99-11687-9991-104
Average (%)1079398
1:8Range (%)97-11381-9392-109
Average (%)1048899
1:16Range (%)96-11285-9895-107
Average (%)10390100
Recovery:
Sample TypeRange (%)Average Recovery (%)
EDTA Plasma (n=8)94-10699
Cell Culture Media (n=8)93-10799
Precision:
Intra-assay PrecisionInter-assay Precision
Sample123123
n20.020.020.020.020.020.0
Mean (ng/mL)24.2377.58217.5825.8970.18231.39
Standard deviation1.273.5113.271.565.7415.18
C V (%)5.254.536.16.038.186.56
Uniport ID:P01375
Sample type & Sample volume:Serum, Plasma And Other Biological Fluids; 100 μL
Specificity:This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed
Storage:2-8℃
Research Area:Cancer,Cardiovascular,Metabolism,Immunology,Signal Transduction
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