Human TLR4 ELISA Kit (HUFI00788)
- SKU:
- HUFI00788
- Product Type:
- ELISA Kit
- Uniprot:
- O00206
- ELISA Type:
- Sandwich
- Synonyms:
- TLR4, CD284, ARMD10, CD284 antigen, hTollhomolog of Drosophila toll, TOLL, toll-like receptor 4
- Reactivity:
- Human
- Research Area:
- Immunology
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 0.188 ng/mL
- Range:
- 0.313-20 ng/mL
Description
Human TLR4 ELISA Kit
The Human TLR4 ELISA Kit is a reliable and precise assay designed for the quantitative determination of Toll-like receptor 4 (TLR4) levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research applications. TLR4 is a key component of the innate immune system, involved in recognizing pathogen-associated molecular patterns and triggering immune responses. Dysregulation of TLR4 signaling has been linked to a variety of inflammatory diseases, autoimmune disorders, and infectious diseases.
Therefore, measuring TLR4 levels can provide valuable insights into the immune response and potential therapeutic targets. With its user-friendly protocol and reliable performance, the Human TLR4 ELISA Kit is a valuable tool for researchers studying immune responses, inflammation, and infectious diseases. Order yours today to advance your research and discovery efforts in the field of immunology.
| Product Name: | Human TLR4 ELISA Kit |
| SKU: | HUFI00788 |
| Reactivity: | Human |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 0.188 ng/mL |
| Range: | 0.313-20 ng/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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