Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
The Human TIMP-1 (Tissue Inhibitors of Metalloproteinase-1) ELISA Kit is a powerful tool for the precise measurement of TIMP-1 levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results, making it suitable for various research purposes.TIMP-1 is a key protein that regulates the activity of metalloproteinases, which are enzymes involved in tissue remodeling and cell migration. Dysregulation of TIMP-1 has been linked to various diseases, including cancer, arthritis, and cardiovascular disorders, highlighting its importance as a potential therapeutic target and diagnostic marker. With the Human TIMP-1 ELISA Kit, researchers can effectively study the role of TIMP-1 in disease pathogenesis and progression, leading to valuable insights for developing novel treatment strategies. Order your kit today from AssayGenie and accelerate your research efforts in the field of metalloproteinase regulation.
Product Name:
Human TIMP-1 (Tissue Inhibitors of Metalloproteinase 1) ELISA Kit
SKU:
HUES01451
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
