Human TGF- alpha (Transforming Growth Factor alpha) ELISA Kit
The Human TGF-Alpha (Transforming Growth Factor-Alpha) ELISA Kit is specifically designed for the precise quantification of TGF-Alpha levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it a valuable tool for various research applications.TGF-Alpha is a key growth factor that plays a crucial role in cell proliferation, differentiation, and migration. It is involved in numerous physiological processes, including tissue development, wound healing, and immune response regulation. Dysregulation of TGF-Alpha signaling has been linked to various diseases, such as cancer, fibrosis, and inflammatory disorders, highlighting its importance as a biomarker for studying and potentially treating these conditions.Overall, the Human TGF-Alpha ELISA Kit provides researchers with a robust and efficient method for detecting and measuring TGF-Alpha levels, leading to a better understanding of its role in health and disease.
Product Name:
Human TGF- alpha (Transforming Growth Factor alpha) ELISA Kit
SKU:
HUES02596
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
