Human SORD/Sorbitol Dehydrogenase ELISA Kit (HUFI01394)

Human SORD/Sorbitol Dehydrogenase ELISA Kit

The Human SORD (Sorbitol Dehydrogenase) ELISA Kit is specifically designed for the precise measurement of sorbitol dehydrogenase levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes. Sorbitol dehydrogenase is a key enzyme involved in the metabolism of sorbitol, playing a crucial role in sorbitol-induced osmotic stress and diabetic complications.

Dysregulation of sorbitol dehydrogenase has been implicated in various diseases, including diabetes and diabetic retinopathy, underscoring its significance as a biomarker for studying these conditions and exploring potential therapeutic interventions. Overall, the Human SORD ELISA Kit provides researchers with a valuable tool for investigating the role of sorbitol dehydrogenase in disease pathogenesis and developing innovative strategies for disease management.

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.