Human Cell Biology ELISA Kits 1
Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit (HUES02954)
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- SKU:
- HUES02954
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P20138
- ELISA Type:
- Sandwich
- Synonyms:
- CD33, SIGLEC-3, gp67
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
- Assay Type:
- Sandwich-ELISA
- Sensitivity:
- 0.1 ng/mL
- Range:
- 0.16-10 ng/mL
- Recovery:
- 80%-120%
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Description
Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit
The Human SIGLEC3 (Sialic acid-binding Ig-like lectin 3) ELISA Kit is specially designed for the precise measurement of SIGLEC3 levels in human serum, plasma, and cell culture supernatants. This kit offers excellent sensitivity and specificity, ensuring dependable and consistent results for various research purposes.SIGLEC3 is a key protein involved in immune responses and cell signaling. It plays a crucial role in regulating inflammatory processes and immune cell activation. Dysregulation of SIGLEC3 has been linked to autoimmune diseases, cancer, and infectious diseases, making it a valuable biomarker for studying these conditions and developing potential treatments.Overall, the Human SIGLEC3 ELISA Kit provides researchers with a powerful tool for studying the role of SIGLEC3 in various physiological and pathological processes, offering valuable insights for potential therapeutic interventions.
| Product Name: | Human SIGLEC3 (Sialic Acid Binding Ig Like Lectin 3) ELISA Kit |
| SKU: | HUES02954 |
| Size: | 96 Assays |
| Detection Method: | Colorimetric method, ELISA, Sandwich |
| Assay type: | Sandwich-ELISA |
| Assay time: | 3 h 30 min |
| Sensitivity: | 0.1 ng/mL |
| Detection range: | 0.16-10 ng/mL |
| Reovery: | 80%-120% |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
| Component | Specification | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T | -20°C, 12 months |
| Reference Standard | 96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials | -20°C, 12 months |
| Concentrated Biotinylated Detection Ab(100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C, 12 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C (Protect from light), 12 months |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL | 2–8°C, 12 months |
| Biotinylated Detection Ab Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| HRP Conjugate Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL | 2–8°C, 12 months |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C |
| Plate Sealer | 96T/48T/24T: 5 pieces | 96T*5: 25 pieces | 2–8°C |
| Technical Manual | 1 copy | - |
| Certificate of Analysis | 1 copy | - |
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| Uniport ID: | P20138 |
| Sample type & Sample volume: | Serum, Plasma And Other Biological Fluids; 100 μL |
| Specificity: | This kit recognizes Human SIGLEC3 in samples. No significant cross-reactivity or interference between Human SIGLEC3 and analogues was observed |
| Storage: | 2-8℃ |
| Research Area: | Cancer,Immunology,Stem Cells |
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