Human RPS3 Recombinant Protein (RPPB4507)
- SKU:
- RPPB4507
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P23396
Description
| Product Name: | Human RPS3 Recombinant Protein |
| Product Code: | RPPB4507 |
| Size: | 10µg |
| Species: | Human |
| Target: | RPS3 |
| Synonyms: | S3, Ribosomal protein S3, 40S ribosomal protein S3, IMR-90 ribosomal protein S3, MGC87870, FLJ26283, FLJ27450. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The RPS3 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAVQISKKRK FVADGIFKAE LNEFLTRELA EDGYSGVEVR VTPTRTEIII LATRTQNVLG EKGRRIRELT AVVQKRFGFP EGSVELYAEK VATRGLCAIA QAESLRYKLL GGLAVRRACY GVLRFIMESG AKGCEVVVSG KLRGQRAKSM KFVDGLMIHS GDPVNYYVDT AVRHVLLRQG VLGIKVKIML PWDPTGKIGP KKPLPDHVSI VEPKDEILPT TPISEQKGGK PEPPAMPQPV PTA |
40S ribosomal protein S3 is a member of the S3P family of ribosomal proteins. RPS3 facilitates the formation of the mRNA binding site 3' of the codon in the decoding site. Moreover, RPS3 takes part in DNA damage recognition due to its affinity for abasic sites and 7, 8-dihydro-8-oxoguanine residues and its collaboration with human base excision repair proteins OGG1 and Ref-1. Expression of RPS3 is higher in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa.
RPS3 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 263 amino acids (1-243) and having a molecular mass of 28.8 kDa.RPS3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | RPS3: a component of the 40S ribosomal subunit of eukaryotes. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Overexpressed in colorectal cancer. |
| UniProt Protein Details: | Protein type:EC 4.2.99.18; Ribosomal; Translation; RNA-binding; Apoptosis Chromosomal Location of Human Ortholog: 11q13.3-q13.5 Cellular Component: focal adhesion; membrane; mitochondrial inner membrane; cytoplasm; nucleolus; spindle; ribonucleoprotein complex; nucleus; cytosol Molecular Function:mRNA binding; NF-kappaB binding; protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; structural constituent of ribosome; protein kinase A binding; oxidized purine base lesion DNA N-glycosylase activity; iron-sulfur cluster binding; damaged DNA binding; protein kinase binding Biological Process: SRP-dependent cotranslational protein targeting to membrane; mitosis; viral reproduction; transcription, DNA-dependent; translation; apoptosis; viral infectious cycle; translational termination; DNA repair; DNA catabolic process, endonucleolytic; activation of NF-kappaB transcription factor; regulation of translation; cellular protein metabolic process; translational elongation; cell division; translational initiation; mRNA catabolic process, nonsense-mediated decay; viral transcription; gene expression; response to DNA damage stimulus; negative regulation of DNA repair |
| NCBI Summary: | Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit, where it forms part of the domain where translation is initiated. The protein belongs to the S3P family of ribosomal proteins. Studies of the mouse and rat proteins have demonstrated that the protein has an extraribosomal role as an endonuclease involved in the repair of UV-induced DNA damage. The protein appears to be located in both the cytoplasm and nucleus but not in the nucleolus. Higher levels of expression of this gene in colon adenocarcinomas and adenomatous polyps compared to adjacent normal colonic mucosa have been observed. This gene is co-transcribed with the small nucleolar RNA genes U15A and U15B, which are located in its first and fifth introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012] |
| UniProt Code: | P23396 |
| NCBI GenInfo Identifier: | 417719 |
| NCBI Gene ID: | 6188 |
| NCBI Accession: | P23396.2 |
| UniProt Secondary Accession: | P23396,Q498B5, Q8NI95, B2R7N5, J3KN86, |
| UniProt Related Accession: | P23396 |
| Molecular Weight: | 243 |
| NCBI Full Name: | 40S ribosomal protein S3 |
| NCBI Synonym Full Names: | ribosomal protein S3 |
| NCBI Official Symbol: | RPS3�� |
| NCBI Official Synonym Symbols: | S3�� |
| NCBI Protein Information: | 40S ribosomal protein S3; IMR-90 ribosomal protein S3 |
| UniProt Protein Name: | 40S ribosomal protein S3 |
| Protein Family: | Ribosomal protein |
| UniProt Gene Name: | RPS3�� |
| UniProt Entry Name: | RS3_HUMAN |
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