Human RGS17 Recombinant Protein (RPPB4451)

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SKU:
RPPB4451
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UGC6

Description

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Product Name:Human RGS17 Recombinant Protein
Product Code:RPPB4451
Size:25µg
Species:Human
Target:RGS17
Synonyms:Regulator of G-protein signaling 17, RGS-17, RGSZ2, hRGS17.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The RGS17 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl,1mM DTT and 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MRKRQQSQNE GTPAVSQAPG NQRPNNTCCF CWCCCCSCSC LTVRNEERGE NAGRPTHTTK MESIQVLEEC QNPTAEEVLS WSQNFDKMMK APAGRNLFRE FLRTEYSEEN LLFWLACEDL KKEQNKKVIE EKARMIYEDY ISILSPKEVS LDSRVREVIN RNLLDPNPHM YEDAQLQIYT LMHRDSFPRF LNSQIYKSFV ESTAGSSSES

RGS17 weakens the signaling activity of G-proteins by binding to activated GTP-bound G alpha subunits and performing as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, creating inactive G-protein heterotrimers, which terminates the signal. RGS17 has a vital part in ending the signaling by mu opioid receptors and development of tolerance to opioid analgesic drugs.

RGS17 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 230 amino acids (1-210) and having a molecular mass of 26.5 kDa.The RGS17 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:RGS17: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G- protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins.
UniProt Protein Details:

Protein type:GAPs; GAPs, RGS

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cytoplasm; plasma membrane; nucleus

Molecular Function:GTPase activator activity

Biological Process: positive regulation of GTPase activity

NCBI Summary:This gene encodes a member of the regulator of G-protein signaling family. This protein contains a conserved, 120 amino acid motif called the RGS domain and a cysteine-rich region. The protein attenuates the signaling activity of G-proteins by binding to activated, GTP-bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UGC6
NCBI GenInfo Identifier:21361405
NCBI Gene ID:26575
NCBI Accession:NP_036551
UniProt Secondary Accession:Q9UGC6,Q5TF49, Q8TD61, Q9UJS8,
UniProt Related Accession:Q9UGC6
Molecular Weight:24,359 Da
NCBI Full Name:regulator of G-protein signaling 17
NCBI Synonym Full Names:regulator of G-protein signaling 17
NCBI Official Symbol:RGS17��
NCBI Official Synonym Symbols:RGSZ2; RGS-17; hRGS17��
NCBI Protein Information:regulator of G-protein signaling 17
UniProt Protein Name:Regulator of G-protein signaling 17
Protein Family:Regulator of G-protein signaling
UniProt Gene Name:RGS17��
UniProt Entry Name:RGS17_HUMAN
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