Human RGS14 Recombinant Protein (RPPB4449)

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SKU:
RPPB4449
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O43566

Description

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Product Name:Human RGS14 Recombinant Protein
Product Code:RPPB4449
Size:10µg
Species:Human
Target:RGS14
Synonyms:Regulator of G-protein signaling 14, RGS14.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:RGS14 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer, pH7.5, 10% glycerol, 1mM DTT and 200mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 80% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPGKPKHLGV PNGRMVLAVS DGELSSTTGP QGQGEGRGSS LSIHSLPSGP SSPFPTEEQP VASWALSFER LLQDPLGLAY FTEFLKKEFS AENVTFWKAC ERFQQIPASD TQQLAQEARN IYQEFLSSQA LSPVNIDRQA WLGEEVLAEP RPDMFRAQQL QIFNLMKFDS YARFVKSPLY RECLLAEAEG RPLREPGSSR LGSPDATRKK PKLKPGKSLP LGVEELGQLP PVEGPGGRPL RKSFRRELGG TANAALRRES QGSLNSSASL DLGFLAFVSS KSESHRKSLG STEGESESRP GKYCCVYLPD GTASLALARP GLTIRDMLAG ICEKRGLSLPDIKVYLVGNE QALVLDQDCT VLADQEVRLE NRITFELELT ALERVVRISA KPTKRLQEAL QPILEKHGLS PLEVVLHRPG EKQPLDLGKL VSSVAAQRLV LDTLPGVKIS KARDKSPCRS QGCPPRTQDK ATHPPPASPS SLVKVPSSAT GKRQTCDIEG LVELLNRVQS SGAHDQRGLLRKEDLVLPEF LQLPAQGPSS EETPPQTKSA AQPIGGSLNS TTDSAL

Regulator of G-protein signaling 14 (RGS14) is a member of the regulator of G protein signaling family. RGS14 contains one RGS domain, 2 Raf-like Ras-binding domains (RBDs) and one GoLoco motif. RGS14 reduces the signaling activity of G-proteins by binding, via its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. RGS14 is a scaffolding protein which integrates G protein and H-Ras/ERK/MAP kinase signaling pathways, thus making it suitably positioned to repress plasticity in CA2 neurons. Since RGS14 is highly enriched in CA2 pyramidal neurons, it has a role in inhibition of both synaptic plasticity at these synapses and hippocampal-based learning and memory.

RGS14 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 586 amino acids (1-566 a.a) and having a molecular mass of 63.6kDa.RGS14 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:RGS14: Acts as a regulator of G protein signaling (RGS). Modulates G protein alpha subunits nucleotide exchange and hydrolysis activities by functioning either as a GTPase-activating protein (GAP), thereby driving G protein alpha subunits into their inactive GDP-bound form, or as a GDP-dissociation inhibitor (GDI). Confers GDI activity on G(i) alpha subunits GNAI1 and GNAI3, but not G(o) alpha subunit GNAO1 and G(i) alpha subunit GNAI2. Confers GAP activity on G(o) alpha subunit GNAI0 and G(i) alpha subunits GNAI2 and GNAI3. May act as a scaffold integrating G protein and Ras/Raf MAPkinase signaling pathways. Inhibits platelet-derived growth factor (PDGF)-stimulated ERK1/ERK2 phosphorylation; a process depending on its interaction with HRAS1 and that is reversed by G(i) alpha subunit GNAI1. Acts as a positive modulator of microtubule polymerisation and spindle organization through a G(i)-alpha-dependent mechanism. Plays a role in cell division. Probably required for the nerve growth factor (NGF)-mediated neurite outgrowth. May be involved in visual memory processing capacity and hippocampal-based learning and memory. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 5q35.3

Cellular Component: centrosome; cytoplasm; dendrite; dendritic spine; nuclear body; nucleus; plasma membrane; postsynaptic density; spindle; spindle pole

Molecular Function:GDP-dissociation inhibitor activity; GTPase activator activity; microtubule binding; protein binding; receptor signaling complex scaffold activity; receptor signaling protein activity

Biological Process: cell division; chromosome segregation; learning; long-term memory; negative regulation of MAP kinase activity; negative regulation of synaptic plasticity; nucleocytoplasmic transport; platelet-derived growth factor receptor signaling pathway; positive regulation of neurogenesis; regulation of transcription in response to stress; response to oxidative stress; spindle organization and biogenesis; visual learning; zygote asymmetric cell division

NCBI Summary:This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]
UniProt Code:O43566
NCBI GenInfo Identifier:257051088
NCBI Gene ID:10636
NCBI Accession:O43566.4
UniProt Secondary Accession:O43566,O43565, Q506M1, Q6ZWA4, Q8TD62,
UniProt Related Accession:O43566
Molecular Weight:36,922 Da
NCBI Full Name:Regulator of G-protein signaling 14
NCBI Synonym Full Names:regulator of G-protein signaling 14
NCBI Official Symbol:RGS14��
NCBI Protein Information:regulator of G-protein signaling 14
UniProt Protein Name:Regulator of G-protein signaling 14
Protein Family:Regulator of G-protein signaling
UniProt Gene Name:RGS14��
UniProt Entry Name:RGS14_HUMAN
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