Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit
The Human Rac1 (Ras-related C3 botulinum toxin substrate 1) ELISA Kit is specifically designed for the accurate measurement of Rac1 levels in human samples, including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit provides reliable and reproducible results, making it perfect for a variety of research applications.Rac1 is a key protein involved in cell signaling pathways, regulating processes such as cell growth, migration, and invasion. Dysregulation of Rac1 has been implicated in numerous diseases, including cancer, cardiovascular disorders, and neurological conditions, making it an important biomarker for studying these diseases and developing potential therapies.Order the Human Rac1 ELISA Kit today and uncover valuable insights into Rac1 activity in human samples, advancing your research and contributing to the understanding of various disease mechanisms.
Product Name:
Human Rac1 (Ras Related C3 Botulinum Toxin Substrate 1) ELISA Kit
SKU:
HUES02572
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
