Human Pulmonary Surfactant Associated Protein A/SPA ELISA Kit (HUFI02867)
- SKU:
- HUFI02867
- Product Type:
- ELISA Kit
- Uniprot:
- Q8IWL2
- ELISA Type:
- Sandwich
- Synonyms:
- SPA, Pulmonary Surfactant Associated Protein A, SFTPA1, COLEC4, PSAP, SFTP1, SFTPA, SFTPA1B, PSP-A, PSPA, SP-A, SP-A1, Collectin-4, 35 kDa pulmonary surfactant-associated protein, Alveolar proteinosis protein
- Reactivity:
- Human
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 9.375 pg/mL
- Range:
- 15.625-1000 pg/mL
Description
Human Pulmonary Surfactant Associated Protein A/SPA ELISA Kit
The Human Pulmonary Surfactant-Associated Protein A (SPA) ELISA Kit is specifically designed for the precise quantification of SPA levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing dependable and reproducible results, making it an indispensable tool for a diverse range of research applications. SPA is a vital component of pulmonary surfactant, a substance that reduces surface tension in the lungs and facilitates proper lung function.
SPA plays a crucial role in immune defense mechanisms in the lungs, protecting against infections and maintaining respiratory health. Abnormal levels of SPA have been linked to various respiratory diseases, making it a valuable biomarker for studying these conditions and potential therapeutic interventions. Overall, the Human Pulmonary Surfactant-Associated Protein A (SPA) ELISA Kit provides researchers with a reliable and accurate method for studying SPA levels and understanding its role in pulmonary health and disease.
| Product Name: | Human Pulmonary Surfactant Associated Protein A/SPA ELISA Kit |
| SKU: | HUFI02867 |
| Reactivity: | Human |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 9.375 pg/mL |
| Range: | 15.625-1000 pg/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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