Human Proteinase-activated receptor 4/PAR4 ELISA Kit (HUFI01999)
- SKU:
- HUFI01999
- Product Type:
- ELISA Kit
- Uniprot:
- Q96RI0
- ELISA Type:
- Sandwich
- Synonyms:
- F2RL3, Coagulation factor II receptor-like 3, Thrombin receptor-like 3,
- Reactivity:
- Human
- Research Area:
- Cardiovascular
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 0.469 ng/mL
- Range:
- 0.781-50 ng/mL
Description
Human Proteinase-activated receptor 4/PAR4 ELISA Kit
The Human Proteinase-Activated Receptor 4 (PAR4) ELISA Kit is a highly sensitive and specific assay designed for the accurate detection of PAR4 levels in human serum, plasma, and cell culture supernatants. This kit provides reliable and reproducible results, making it suitable for various research applications. Proteinase-activated receptor 4 (PAR4) is a key player in the regulation of platelet activation and function, making it an important target for studying thrombosis and other cardiovascular disorders.
By accurately measuring PAR4 levels, researchers can gain valuable insights into the mechanisms underlying these conditions and potentially identify novel therapeutic targets. Whether investigating cardiovascular diseases, coagulation disorders, or other related conditions, the Human PAR4 ELISA Kit offers a convenient and reliable solution for studying the role of PAR4 in human health and disease. Trust AssayGenie for high-quality ELISA kits that empower your research endeavors.
| Product Name: | Human Proteinase-activated receptor 4/PAR4 ELISA Kit |
| SKU: | HUFI01999 |
| Reactivity: | Human |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 0.469 ng/mL |
| Range: | 0.781-50 ng/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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