Protein Phosphatase 1A (PPM1A) is a member of the PP2C family of Ser/Thr protein phosphatases which are known to be negative regulators of cell stress response pathways. PPM1A has a broad specificity. PPM1A negatively regulates the activities of MAP kinases and MAP kinase kinases. Also; it negatively regulates TGF-beta signaling through dephosphorylating SMAD2 and SMAD3; resulting in their dissociation from SMAD4; nuclear export of the SMADs and termination of the TGF-beta-mediated signaling. In addition; PPM1A can dephosphorylate cyclin-dependent kinases; and thus may be involved in cell cycle control.
Product Name:
Human PPM1A Recombinant Protein (RPES1769)
Product Code:
RPES1769
Size:
10µg
Species:
Human
Expressed Host:
E.coli
Synonyms:
Protein Phosphatase 1A, Protein Phosphatase 2C Isoform Alpha, PP2C-Alpha, Protein Phosphatase IA, PPM1A, PPPM1A,PP2C-ALPHA,PP2CA,PP2Calpha
Accession:
P35813-1
Sequence:
Gly2-Trp382
Fusion tag:
C-6His
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Protein Phosphatase 1A is produced by our E.coli expression system and the target gene encoding Gly2-Trp382 is expressed with a 6His tag at the C-terminus.
Purity:
> 90 % as determined by reducing SDS-PAGE.
Mol Mass:
43.5 kDa
AP Mol Mass:
45 kDa
Formulation:
Supplied as a 0.2 µm filtered solution of 20mM PB; 150mM NaCl; pH 7.4.
Shipping:
This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs.Upon receipt; store it immediately at<-20°C.
Stability and Storage:
Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
