Human Phosphotyrosine MCSFR PharmaGenie ELISA Kit (SBRS1889)

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SKU:
SBRS1889
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human Phosphotyrosine MCSFR PharmaGenie ELISA Kit (SBRS1889)

Assay Genie Phospho-M-CSF R ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phospho-M-CSF R in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-M-CSF R. An anti-M-CSF R antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated and unphosphorylated M-CSF R present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-phosphotyrosine antibody is used to detect only tyrosine-phosphorylated protein. After washing away unbound antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of phospho-M-CSF R bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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