Human Phospho-ZAP70 (Y493) and Total ZAP70 PharmaGenie ELISA Kit (SBRS2021)

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SKU:
SBRS2021
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human Phospho-ZAP70 (Y493) and Total ZAP70 PharmaGenie ELISA Kit (SBRS2021)

Assay Genie Phospho- ZAP70 (Y493) and Total ZAP70 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated ZAP70 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-ZAP70 and total ZAP70. An anti-pan ZAP70 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and ZAP70 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-ZAP70 (Y493) antibody is used to detect phosphorylated ZAP70 or rabbit anti-ZAP70 antibody is used to detect pan ZAP70. After washing away unbound antibody, HRP-conjugated streptavidin or HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ZAP70 (Y493) or pan ZAP70 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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