Human Phospho-HSP27 (S82) and Total HSP27 PharmaGenie ELISA Kit (SBRS1853)

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SKU:
SBRS1853
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

Human Phospho-HSP27 (S82) and Total HSP27 PharmaGenie ELISA Kit (SBRS1853)

Assay Genie Phospho- HSP27 (S82) and Total HSP27 ELISA kit is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated HSP27 protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-HSP27 and total HSP27. An anti-pan HSP27 antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and HSP27 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti-HSP27 (S82) antibody is used to detect phosphorylated HSP27 or biotinylated anti-HSP27 antibody is used to detect pan HSP27. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG or HRP-conjugated Streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of HSP27 (S82) or pan HSP27 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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