The Human NRG-1 (Neuregulin-1) CLIA Kit is a highly accurate assay designed to measure levels of Neuregulin-1 in human samples including serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring dependable and consistent results for various research applications.Neuregulin-1 is a critical protein involved in regulating cell growth, differentiation, and survival in various cell types, particularly in the nervous system. Dysregulation of Neuregulin-1 has been linked to various diseases, including schizophrenia, bipolar disorder, and certain types of cancer, underscoring its importance as a biomarker for disease research and potential therapeutic development.
The Human NRG-1 CLIA Kit from Assay Genie offers a reliable and efficient solution for studying the role of Neuregulin-1 in health and disease, enabling researchers to gain valuable insights into its function and potential clinical applications.
Product Name:
Human NRG-1 (Neuregulin 1) CLIA Kit
SKU:
HUES01041
Target:
Human NRG-1 (Neuregulin 1)
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human NRG-1. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human NRG-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human NRG-1, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human NRG-1. You can calculate the concentration of Human NRG-1 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-109
99-114
92-108
Average (%)
102
104
99
1:4
Range (%)
98-114
94-106
88-98
Average (%)
105
100
93
1:8
Range (%)
90-102
84-98
102-117
Average (%)
95
91
108
1:16
Range (%)
91-104
101-114
90-105
Average (%)
97
108
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-112
103
EDTA plasma (n=5)
97-114
104
Cell culture media (n=5)
89-102
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
94.05
218.77
834.79
87.98
199.8
768.9
Standard deviation
10.4
16.63
52.09
8.09
17.7
78.2
C V (%)
11.06
7.6
6.24
9.2
8.86
10.17
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human NRG-1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human NRG-1 in samples. No significant cross-reactivity or interference between Human NRG-1 and analogues was observed.
