Human Noggin/NOG Recombinant Protein (RPES4959)
- SKU:
- RPES4959
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
Human Noggin/NOG Recombinant Protein
Noggin is a secreted protein involved at multiple stages of vertebrate embryonic development including neural induction and is known to exert its effects by inhibiting the bone morphogenetic protein (BMP)-signaling pathway. It binds several BMPs with very high (picomolar) affinities; with a marked preference for BMP2 and BMP4 over BMP7. By binding tightly to BMPs; Noggin prevents BMPs from binding their receptors. Noggin binds the bone morphogenetic proteins (BMP) such as BMP-4 and BMP-7; and inhibits BMP signaling by blocking the molecular interfaces of the binding epitopes for both type I and type II receptors. Interaction of BMP and its antagonist Noggin governs various developmental and cellular processes; including embryonic dorsal-ventral axis; induction of neural tissue; formation of joints in the skeletal system and neurogenesis in the adult brain. Noggin plays a key role in neural induction by inhibiting BMP4; along with other TGF-β signaling inhibitors such as chordin and follistatin. Mouse knockout experiments have demonstrated that noggin also plays a crucial role in bone development; joint formation; and neural tube fusion.
| Product Name: | Human Noggin/NOG Recombinant Protein (RPES4959) |
| Product Code: | RPES4959 |
| Size: | 50µg |
| Species: | Human |
| Expressed Host: | HEK293 Cells |
| Synonyms: | Noggin,SYM1,SYNS1 |
| Accession: | NP_005441.1 |
| Sequence: | Met 1-Cys 232 |
| Fusion tag: | C-Fc |
| Activity: | 1. Measured by its ability to inhibit BMP2-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 1.5-2.0 µg /mL in the presence of 0.25-0.5 µg/mL of BMP-2.2. Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3-E1 cells.The ED50 for this effect is typically 0.1-0.6 µg/mL in the presence of 50 ng/mL of hBMP4. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human Noggin precursor (NP_005441.1) (Met 1-Cys 232) was fused with the Fc region of human IgG1 at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 49.8 kDa |
| AP Mol Mass: | 58-62 kDa |
| Formulation: | Lyophilized from sterile 100mM Glycine; 10mM NaCl; 50mM Tris; pH 7.5 |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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