Neurotrophins Recombinant Proteins

Human NNT1 Recombinant Protein (RPPB0784)

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SKU:
RPPB0784
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UBD9
Research Area:
Neurotrophins

Description

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Product Name:Human NNT1 Recombinant Protein
Product Code:RPPB0784
Size:10µg
Species:Human
Target:NNT1
Synonyms:Cardiotrophin-Like Cytokine Factor 1, Novel Neurotrophin-1 , BSF3, CLC, CRLF1 Associated Cytokine-Like Factor 1, B-Cell Stimulating Factor 3, B-Cell-Stimulating Factor 3, BSF-3, NNT-1, NNT1, Neurotrophin-1/B-Cell Stimulating Factor-3, Cold-Induced Sweating Syndrome 2, B-Cell Stimulatory Factor 3, CISS2, NR6, CLCF1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:NNT-1 protein was lyophilized from a 0.2�m filtered solution in Acetonitrile and TFA.
Solubility:It is recommended to reconstitute the lyophilized NNT1 in sterile 18M-cm H2O not less than 0.5mg/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized Neurotrophin-1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NNT1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MLNRTGDPGP GPSIQKTYDL TRYLEHQLRS LAGTYLNYLG PPFNEPDFNP PRLGAETLPR ATVDLEVWRS LNDKLRLTQN YEAYSHLLCY LRGLNRQAAT AELRRSLAHF CTSLQGLLGS IAGVMAALGY PLPQPLPGTE PTWTPGPAHS DFLQKMDDFW LLKELQTWLW RSAKDFNRLK KKMQPPAAAV TLHLGAHGF
Biological Activity:The ED50, as measured in a cell proliferation assay using human TF?1 cells transfected with human CNTF R?, is less than 15ng/ml.

Cardiotrophin-like cytokine (CLC/ NNT-1) belongs to the IL-6 family of cytokines. All family members share the receptor subunit gp130, which belongs to the type I cytokine receptor superfamily. NNT1 is a trophic factor for motor neurons, a stimulator of ACTH release from corticotrophs, and an inducer of IgE synthesis and B cell proliferation. Cells expressing NNT-1 include embryonic muscle, lung epithelium, and mesenchyme. NNT1 binds to and activates the ILST/gp130 receptor.

Neurotrophin-1 Human Recombinant (28-225) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 199 amino acids and having a molecular mass of 22kDa.The NNT-1 is purified by proprietary chromatographic techniques.

UniProt Protein Function:CLCF1: Cytokine with B-cell stimulating capability. Binds to and activates the ILST/gp130 receptor. Defects in CLCF1 are the cause of cold-induced sweating syndrome type 2 (CISS2). Cold-induced sweating syndrome (CISS) is an autosomal recessive disorder characterized by profuse sweating induced by cool surroundings (temperatures of 7 to 18 degrees Celsius). Additional abnormalities include a high- arched palate, nasal voice, depressed nasal bridge, inability to fully extend the elbows and kyphoscoliosis. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Apoptosis; Cytokine; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 11q13.3

Cellular Component: extracellular region

Molecular Function:ciliary neurotrophic factor receptor binding; cytokine activity; growth factor activity; protein binding; protein heterodimerization activity; receptor binding

Biological Process: B cell differentiation; cell surface receptor linked signal transduction; JAK-STAT cascade; negative regulation of neuron apoptosis; positive regulation of astrocyte differentiation; positive regulation of B cell proliferation; positive regulation of cell proliferation; positive regulation of immunoglobulin production; positive regulation of isotype switching to IgE isotypes; positive regulation of tyrosine phosphorylation of Stat3 protein

Disease: Cold-induced Sweating Syndrome 2

NCBI Summary:This gene is a member of the glycoprotein (gp)130 cytokine family and encodes cardiotrophin-like cytokine factor 1 (CLCF1). CLCF1 forms a heterodimer complex with cytokine receptor-like factor 1 (CRLF1). This dimer competes with ciliary neurotrophic factor (CNTF) for binding to the ciliary neurotrophic factor receptor (CNTFR) complex, and activates the Jak-STAT signaling cascade. CLCF1 can be actively secreted from cells by forming a complex with soluble type I CRLF1 or soluble CNTFR. CLCF1 is a potent neurotrophic factor, B-cell stimulatory agent and neuroendocrine modulator of pituitary corticotroph function. Defects in CLCF1 cause cold-induced sweating syndrome 2 (CISS2). This syndrome is characterized by a profuse sweating after exposure to cold as well as congenital physical abnormalities of the head and spine. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Oct 2009]
UniProt Code:Q9UBD9
NCBI GenInfo Identifier:56404673
NCBI Gene ID:23529
NCBI Accession:Q9UBD9.1
UniProt Secondary Accession:Q9UBD9,Q6NZA4, B4DNT4,
UniProt Related Accession:Q9UBD9
Molecular Weight:24,087 Da
NCBI Full Name:Cardiotrophin-like cytokine factor 1
NCBI Synonym Full Names:cardiotrophin-like cytokine factor 1
NCBI Official Symbol:CLCF1��
NCBI Official Synonym Symbols:CLC; NR6; BSF3; NNT1; BSF-3; CISS2; NNT-1��
NCBI Protein Information:cardiotrophin-like cytokine factor 1
UniProt Protein Name:Cardiotrophin-like cytokine factor 1
UniProt Synonym Protein Names:B-cell-stimulating factor 3; BSF-3; Novel neurotrophin-1; NNT-1
Protein Family:Cardiotrophin-like cytokine factor
UniProt Gene Name:CLCF1��
UniProt Entry Name:CLCF1_HUMAN
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