The Human NES1 (Nesfatin-1) ELISA Kit is specifically designed for the precise detection of Nesfatin-1 levels in human serum, plasma, and cell culture supernatants. This advanced kit boasts exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.Nesfatin-1 is a key peptide hormone that plays a crucial role in regulating appetite, energy metabolism, and body weight. It is also involved in various physiological processes such as stress response, sleep regulation, and reproductive function. Understanding Nesfatin-1 levels can provide valuable insights into conditions like obesity, diabetes, and eating disorders, making it a valuable biomarker for exploring these conditions and developing potential treatments.Overall, the Human NES1 (Nesfatin-1) ELISA Kit is an essential tool for researchers looking to investigate the role of Nesfatin-1 in human physiology and pathology, offering reliable and precise measurements to support their studies.
Product Name:
Human NES1 (Nesfatin 1) ELISA Kit
SKU:
HUES03244
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
