Human NDUFS8/NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit (HUFI01535)
![Human NDUFS8/NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit (HUFI01535)](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/608x608/products/1042/11680/human-ndufs8-nadh-dehydrogenase-ubiquinone-iron-sulfur-protein-8-mitochondrial-elisa-kit__69720__69114.1706266263.jpg?c=1 "Human NDUFS8/NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit (HUFI01535)")
- SKU:
- HUFI01535
- Product Type:
- ELISA Kit
- Uniprot:
- O00217
- ELISA Type:
- Sandwich
- Synonyms:
- NDUFS8, NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial, TYKY subunit, Complex I-23kD, CI-23kD, NADH-ubiquinone oxidoreductase 23 kDa subunit, TYKY, CI-23k, CI23KD
- Reactivity:
- Human
- Research Area:
- Cell Biology
- Size:
- 48T/96T
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 0.188 ng/mL
- Range:
- 0.313-20 ng/mL
![Human NDUFS8/NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit (HUFI01535)](https://cdn11.bigcommerce.com/s-rd6ounxcu2/images/stencil/1280x1280/products/1042/11680/human-ndufs8-nadh-dehydrogenase-ubiquinone-iron-sulfur-protein-8-mitochondrial-elisa-kit__69720__69114.1706266263.jpg?c=1&imbypass=on "Human NDUFS8/NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit (HUFI01535)")
Description
Human NDUFS8/NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit
The Human NDUFS8 (NADH dehydrogenase [ubiquinone] iron-sulfur protein 8) Mitochondrial ELISA Kit is specifically designed for the accurate quantification of NDUFS8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications. NDUFS8 is a critical component of the mitochondrial respiratory chain complex I, playing a key role in electron transport and ATP production.
Dysregulation of NDUFS8 has been implicated in various mitochondrial disorders and neurodegenerative diseases, underscoring its importance as a biomarker for studying these conditions and potential therapeutic interventions. With the Human NDUFS8 Mitochondrial ELISA Kit, researchers can confidently measure NDUFS8 levels in human samples, providing valuable insights into mitochondrial function and disease pathology. Upgrade your research capabilities with this cutting-edge ELISA kit from Assay Genie.
| Product Name: | Human NDUFS8/NADH dehydrogenase [ubiquinone] iron-sulfur protein 8, mitochondrial ELISA Kit |
| SKU: | HUFI01535 |
| Reactivity: | Human |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 0.188 ng/mL |
| Range: | 0.313-20 ng/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
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| Recovery: |
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Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
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