Human NDUFA6 ELISA Kit (HUFI01344)

Human NDUFA6 ELISA Kit

The Human NDUFA6 ELISA Kit is specifically designed for the precise measurement of NDUFA6 levels in human samples. This kit offers high sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research purposes. NDUFA6, also known as NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6, is a key component of the mitochondrial respiratory chain complex I. Dysfunction or mutations in NDUFA6 have been linked to various diseases, including mitochondrial disorders and neurodegenerative conditions, highlighting its importance as a potential biomarker for studying these conditions and developing therapeutic interventions.

With its reliable performance and broad applicability, the Human NDUFA6 ELISA Kit is an indispensable tool for researchers investigating the role of NDUFA6 in health and disease.

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.