Human Cell Biology ELISA Kits 1
Human mTOR (Mammalian Target of Rapamycin) ELISA Kit (HUES02655)
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- SKU:
- HUES02655
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P42345
- ELISA Type:
- Sandwich
- Synonyms:
- FRAP1, RAFT1, RAPT1, Mechanistic target of rapamycin, FK506 binding protein 12-rapamycin associated protein 1
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
- Assay Type:
- Sandwich-ELISA
- Sensitivity:
- 0.1 ng/mL
- Range:
- 0.16-10 ng/mL
- Recovery:
- 80%-120%
Frequently bought together:
Description
Human mTOR (Mammalian Target of Rapamycin) ELISA Kit
The Human mTOR (Mammalian Target of Rapamycin) ELISA Kit is specifically designed for the accurate detection of mTOR levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.mTOR is a key protein involved in regulating cell growth, metabolism, and survival, making it a critical target for studying diseases such as cancer, diabetes, and neurological disorders. By accurately measuring mTOR levels, researchers can gain valuable insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human mTOR ELISA Kit from Assay Genie provides researchers with a reliable tool to investigate the role of mTOR in various physiological and pathological processes, paving the way for novel discoveries and advancements in biomedical research.
| Product Name: | Human mTOR (Mammalian Target of Rapamycin) ELISA Kit |
| SKU: | HUES02655 |
| Size: | 96 Assays |
| Detection Method: | Colorimetric method, ELISA, Sandwich |
| Assay type: | Sandwich-ELISA |
| Assay time: | 3 h 30 min |
| Sensitivity: | 0.1 ng/mL |
| Detection range: | 0.16-10 ng/mL |
| Reovery: | 80%-120% |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
| Component | Specification | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T | -20°C, 12 months |
| Reference Standard | 96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials | -20°C, 12 months |
| Concentrated Biotinylated Detection Ab(100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C, 12 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C (Protect from light), 12 months |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL | 2–8°C, 12 months |
| Biotinylated Detection Ab Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| HRP Conjugate Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL | 2–8°C, 12 months |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C |
| Plate Sealer | 96T/48T/24T: 5 pieces | 96T*5: 25 pieces | 2–8°C |
| Technical Manual | 1 copy | - |
| Certificate of Analysis | 1 copy | - |
| Linearity: |
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| Uniport ID: | P42345 |
| Sample type & Sample volume: | Serum, Plasma And Other Biological Fluids; 100 μL |
| Specificity: | This kit recognizes Human mTOR in samples. No significant cross-reactivity or interference between Human mTOR and analogues was observed |
| Storage: | 2-8℃ |
| Research Area: | Cancer,Cell Biology,Cardiovascular,Epigenetics And Nuclear Signaling,Metabolism |
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