Human MMP 9 ELISA Kit
- SKU:
- HUFI00211
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Description
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| Product Name: | Human MMP 9 ELISA Kit |
| Product Code: | HUFI00211 |
| Size: | 96 Assays |
| Alias: | MMP-9, Matrix Metalloproteinase 9, CLG4B, Gelatinase B, GELB, CLG4B, macrophage gelatinase, MANDP2, MMP-9, type V collagenase |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human MMP-9 concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.188ng/ml |
| Range: | 0.313-20ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human MMP-9 and the recovery rates were calculated by comparing the measured value to the expected amount of Human MMP-9 in samples. |
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human MMP-9 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. |
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | P14780 |
| UniProt Protein Function: | MMP9: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly--Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Exists as monomer or homodimer; disulfide-linked. Exists also as Fill out our quote form below and a dedicated member of staff will get back to you within one working day! Additional Information
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