Human MMP 2 ELISA Kit
- SKU:
- HUFI00017
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P08253
- Sensitivity:
- 0.191ng/ml
- Range:
- 0.5-32ng/ml
- ELISA Type:
- Sandwich
- Synonyms:
- MMP-2, Matrix Metalloproteinase 2, Gelatinase A, 72 kDa gelatinase, CLG4, collagenase type IV-A, 72 kDa type IV collagenase
- Reactivity:
- Human
- Research Area:
- Cardiovascular
Description
Human MMP 2 ELISA Kit
MMP 2 is a polymorphonuclear leukocyte protein that is involved in enhancing the immune response through chemotaxis and phagocytosis. MMP 2 can work together with MMP 9 to degrade endothelial cells, thereby enabling MMP2 to be found in dermal fibroblast cells throughout. MMP 2 plays a key role in the degradation of the extracellular matrix during normal physiological processes, such as embryonic development and reproduction. MMP 2 functions in angiogenesis and lymphangiogenesis along with MMP 9. MMP 2 also functions to promote tumor invasion and metastasis. MMP 2 is known to bind several proteins (including MMP 1 and Tissue Factor) that serve as co-factors in the proteolytic cascade. The Assay Genie Human MMP 2 ELISA kit is a highly sensitive assay for the quantitative measurement of MMP 2 in serum, blood, plasma, cell culture supernatant, and tissue samples.
| Product Name: | Human MMP 2 ELISA Kit |
| Product Code: | HUFI00017 |
| Size: | 96 Assays |
| Alias: | MMP-2, Matrix Metalloproteinase 2, Gelatinase A, 72 kDa gelatinase, CLG4, collagenase type IV-A, 72 kDa type IV collagenase |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human MMP-2 concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 0.191ng/ml |
| Range: | 0.5-32ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human MMP-2 and the recovery rates were calculated by comparing the measured value to the expected amount of Human MMP-2 in samples. | ||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human MMP-2 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | P08253 |
| UniProt Protein Function: | MMP2: Ubiquitinous metalloproteinase that is involved in diverse functions such as remodeling of the vasculature, angiogenesis, tissue repair, tumor invasion, inflammation, and atherosclerotic plaque rupture. As well as degrading extracellular matrix proteins, can also act on several nonmatrix proteins such as big endothelial 1 and beta-type CGRP promoting vasoconstriction. Also cleaves KISS at a Gly--Leu bond. Appears to have a role in myocardial cell death pathways. Contributes to myocardial oxidative stress by regulating the activity of GSK3beta. Cleaves GSK3beta in vitro. Interacts (via the C-terminal hemopexin-like domains- containing region) wit Fill out our quote form below and a dedicated member of staff will get back to you within one working day! Related Products Human anti-MMP-2 ELISA Kit
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