Human MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) ELISA Kit
The Human MIP-3 Alpha (Macrophage Inflammatory Protein 3 Alpha) ELISA Kit is a valuable tool for the precise measurement of MIP-3 Alpha levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.MIP-3 Alpha is a key chemokine involved in the recruitment and activation of immune cells, particularly macrophages. It plays a crucial role in inflammatory responses and immune cell trafficking, making it an important target for studying conditions such as inflammatory diseases, autoimmune disorders, and infectious diseases.By using the Human MIP-3 Alpha ELISA Kit, researchers can effectively investigate the role of MIP-3 Alpha in various biological processes and diseases, ultimately contributing to the development of new therapeutic strategies and diagnostic tools.
Product Name:
Human MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) ELISA Kit
SKU:
HUES01314
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
