MHC class I polypeptide-related sequence A, also known as MIC-A, PERB11.1 and MICA, is a single-pass type I membrane protein which belongs to the MHC class I family of MIC subfamily. MICA contains one Ig-like C1-type domain and is expressed on the cell surface, although unlike canonical class I molecules does not seem to associate with beta-2-microglobulin. It is thought that MICA functions as a stress-induced antigen that is broadly recognized by NK cells, NKT cells, and most of the subtypes of T cells. MICA is the ligand for NK cell activating receptor KLRK1/NKG2D. MICA seems to have no role in antigen presentation. MICA leads to cell lysis by binding to KLRK1.
Product Name:
Human MICA Recombinant Protein (RPES4614)
Product Code:
RPES4614
Size:
10µg
Species:
Human
Expressed Host:
Human Cells
Synonyms:
MHC Class I Polypeptide-Related Sequence A, MIC-A, MICA, PERB11.1
Accession:
Q29983
Sequence:
Ala23-Glu308
Fusion tag:
C-Fc
Activity:
Immobilized Human MICA-Fc at 2µg/ml(100 ?l/well) can bind Human NKG2DL2-His(Cat: PKSH032816). The ED50 of Human MICA-Fc is 2.29 ug/ml .
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human MHC Class I Polypeptide-Related Sequence A is produced by our Mammalian expression system and the target gene encoding Ala23-Glu308 is expressed with a Fc tag at the C-terminus.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
59.9 kDa
AP Mol Mass:
88 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of PBS, pH 7.4.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
