Human LOXL2/Lysyl Oxidase Homolog 2 ELISA Kit (HUFI01390)

Human LOXL2/Lysyl Oxidase Homolog 2 ELISA Kit

The Human LOXL2 (Lysyl Oxidase Homolog 2) ELISA Kit is a precise tool for the quantitative measurement of LOXL2 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers accurate and consistent results, making it suitable for a variety of research applications. LOXL2 is a key enzyme involved in the regulation of extracellular matrix remodeling and plays a critical role in processes such as tissue repair, fibrosis, and cancer progression.

Its dysregulation has been linked to various diseases, highlighting its importance as a biomarker for disease diagnosis and therapeutic development. With the Human LOXL2 ELISA Kit, researchers can explore the impact of LOXL2 on disease pathogenesis and treatment outcomes, paving the way for new insights and advancements in the field of biomedical research.

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.