Human JOSD1 Recombinant Protein (RPPB3771)

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SKU:
RPPB3771
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q15040

Description

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Product Name:Human JOSD1 Recombinant Protein
Product Code:RPPB3771
Size:20µg
Species:Human
Target:JOSD1
Synonyms:Josephin-1, Josephin domain-containing 1, JOSD1, JSPH1, KIAA0063, dJ508I15.2.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:JOSD1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 40% glycerol, 5mM DTT and 2mM EDTA.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSCVPWK GDKAKSESLE LPQAAPPQIY HEKQRRELCA LHALNNVFQD SNAFTRDTLQ EIFQRLSPNT MVTPHKKSML GNGNYDVNVI MAALQTKGYE AVWWDKRRDV GVIALTNVMG FIMNLPSSLC WGPLKLPLKR QHWICVREVG GAYYNLDSKL KMPEWIGGES ELRKFLKHHL RGKNCELLLV VPEEVEAHQS WRTDV

Josephin-1 (JOSD1) has minimal protease activity towards poly-ubiquitin chains (in vitro). JOSD1 functions as a deubiquitinating enzyme. Ubiquitin-binding sites in the Josephin domain are a factor in ubiquitin chain binding and cleavage.

JOSD1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 225 amino acids (1-202 a.a.) and having a molecular mass of 25.6kDa.JOSD1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:JOSD1: Has low protease activity towards poly-ubiquitin chains (in vitro). May act as a deubiquitinating enzyme.
UniProt Protein Details:

Protein type:EC 3.4.19.12

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: cytosol; membrane

Molecular Function:protein binding; ubiquitin-specific protease activity

Biological Process: protein deubiquitination

UniProt Code:Q15040
NCBI GenInfo Identifier:3123051
NCBI Gene ID:9929
NCBI Accession:Q15040.1
UniProt Secondary Accession:Q15040,A8K712,
UniProt Related Accession:Q15040
Molecular Weight:23kDa
NCBI Full Name:Josephin-1
NCBI Synonym Full Names:Josephin domain containing 1
NCBI Official Symbol:JOSD1��
NCBI Official Synonym Symbols:dJ508I15.2��
NCBI Protein Information:josephin-1
UniProt Protein Name:Josephin-1
UniProt Synonym Protein Names:Josephin domain-containing protein 1
Protein Family:Josephin
UniProt Gene Name:JOSD1��
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