The Human IL-31 ELISA Kit is specifically designed for the precise measurement of Interleukin-31 (IL-31) levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and consistent results for various research purposes.IL-31 is a key cytokine that plays a critical role in inflammatory responses and immune regulation. Elevated levels of IL-31 have been linked to various inflammatory skin conditions, such as atopic dermatitis and psoriasis, making it a valuable biomarker for studying these diseases and potential therapeutic interventions. With its high performance and reliability, the Human IL-31 ELISA Kit is an essential tool for researchers and clinicians interested in investigating the role of IL-31 in inflammatory disorders and developing targeted treatments.
Product Name:
Human IL-31 ELISA kit
SKU:
HUES03458
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
