The Human IL-21 ELISA Kit is specifically designed for the precise detection of Interleukin-21 (IL-21) levels in human serum, plasma, and cell culture supernatants. With its advanced technology and high sensitivity, this kit ensures accurate and reproducible results, making it a valuable tool for a variety of research applications.IL-21 is a key cytokine that plays a critical role in immune regulation, inflammation, and the development of autoimmune diseases. By measuring IL-21 levels, researchers can gain insights into the immune response and potentially identify new therapeutic targets for various diseases. This ELISA kit provides a simple and efficient method for quantifying IL-21 levels in biological samples, making it a valuable resource for scientists studying immunology, inflammation, and autoimmune disorders. With its reliable performance and ease of use, the Human IL-21 ELISA Kit is a valuable addition to any research laboratory.
Product Name:
Human IL-21 ELISA Kit
SKU:
HUES03314
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
37.5 pg/mL
Detection range:
62.5-4000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
