The Human IL-2 (Interleukin-2) ELISA Kit is specifically designed for the precise measurement of IL-2 levels in human samples including serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring accurate and consistent results for various research purposes.IL-2 is a key cytokine that plays a crucial role in the regulation of immune responses, particularly in T cell activation and proliferation. Dysregulation of IL-2 levels has been linked to autoimmune disorders, infectious diseases, and allergic reactions, highlighting its importance as a biomarker in immunological research and clinical studies.Overall, the Human IL-2 ELISA Kit offers researchers a reliable tool for studying the role of IL-2 in various physiological and pathological processes, aiding in the development of novel therapeutic strategies for immune-related disorders.
Product Name:
Human IL-2 ELISA Kit
SKU:
HUES01385
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
