The Human IL-13 ELISA Kit is specifically designed for the precise measurement of Interleukin-13 (IL-13) levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, guaranteeing accurate and dependable results for various research applications.IL-13 is a key cytokine that plays a crucial role in immune response regulation, particularly in allergic reactions, asthma, and inflammatory diseases. By detecting IL-13 levels, researchers can better understand the underlying mechanisms of these conditions and explore potential therapeutic interventions.With its high-quality components and user-friendly protocols, the Human IL-13 ELISA Kit from Assay Genie is an indispensable tool for scientists and researchers aiming to unravel the complexities of immune system function and inflammatory processes.
Product Name:
Human IL-13 ELISA Kit
SKU:
HUES01390
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
9.38 pg/mL
Detection range:
15.63-1000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
