Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are made by proteolytic degradation of inactive precursor proteins, which include the N- and C-terminal propeptide areas. Mature human IGF-I, which consists of 70 amino acids and has a 94 percent homology with mouse IGF-I, has cross-species activity. IGF-1 interacts with the receptors IGF-IR, IGF-IIR, and the insulin receptor, playing an important role in cell cycle progression and cell proliferation. Growth hormone regulates IGF-1 expression. R3 IGF-1, also known as 83 amino acid IGF-1, is a complete human IGF-1 sequence with the Arg (R) at position three replaced for Glu(E), resulting in R3; and a 13 amino acid extension peptide at the N terminus. The R3 IGF-1 was designed to boost biological activity. In vitro, however, R3 IGF-1 is far more powerful than human IGF-I. Human IGF/IGF1 Recombinant Protein is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
Measured in a serum-free cell proliferation assay using MCF?7 human breast cancer cells. The ED50 for this effect is 14.9 ng/ml.
Endotoxin:
<1.0 EU per µg as determined by the LAL method.
Protein Construction:
Recombinant Human Insulin-like Growth Factor I is produced by our E.coli expression system and the target gene encoding Gly49-Ala118 is expressed.
Purity:
> 95 % as determined by reducing SDS-PAGE.
Mol Mass:
7.6 kDa
AP Mol Mass:
9 kDa
Formulation:
Lyophilized from a 0.2 µm filtered solution of 300mM NaAc; pH 6.5.
Shipping:
This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:
Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
system_update_altDatasheetMouse IGF1/IGF-1/IGF Recombinant Protein IGF I, also known as mechano growth factor, somatomedin-C, IGF-I and IGF1, is a secreted protein which belongs to the?insulin fa
