The Human IFN-Beta (Interferon-Beta) ELISA Kit is a highly reliable and sensitive tool for the precise measurement of IFN-Beta levels in human serum, plasma, and cell culture supernatants. With its high specificity, this kit ensures accurate and reproducible results, making it suitable for a wide range of research applications.IFN-Beta is a key player in the immune response, exerting anti-viral and anti-inflammatory effects by modulating immune cell activity. Dysregulation of IFN-Beta signaling has been implicated in various autoimmune diseases, viral infections, and inflammatory disorders, highlighting its importance as a biomarker in disease research and potential therapeutic development.The Human IFN-Beta ELISA Kit from AssayGenie provides researchers with a valuable tool for studying the role of IFN-Beta in health and disease, offering reliable and precise quantification of this essential cytokine.
Product Name:
Human IFN- beta (Interferon Beta) ELISA Kit
SKU:
HUES01371
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
