Human IFIT1/Interferon-induced protein with tetratricopeptide repeats 1 ELISA Kit (HUFI01406)
- SKU:
- HUFI01406
- Product Type:
- ELISA Kit
- Size:
- 48T/96T
- Reactivity:
- Human
- Detection Method:
- Sandwich ELISA, Double Antibody
- Sensitivity:
- 0.094 ng/mL
- Range:
- 0.156-10 ng/mL
Description
Human IFIT1/Interferon-induced protein with tetratricopeptide repeats 1 ELISA Kit
The Human IFIT1 (Interferon Induced Protein with Tetratricopeptide Repeats 1) ELISA Kit is a powerful tool for detecting and quantifying IFIT1 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it perfect for a variety of research applications. IFIT1 is a key protein that is induced by interferons and plays a critical role in the human immune response to viral infections.
By studying the levels of IFIT1 in different samples, researchers can gain valuable insights into the immune system's antiviral mechanisms and develop novel therapeutic strategies for infectious diseases. Whether you are studying host-virus interactions, immune responses, or developing new antiviral treatments, the Human IFIT1 ELISA Kit from Assay Genie provides a comprehensive solution for accurate IFIT1 detection.
| Product Name: | Human IFIT1/Interferon-induced protein with tetratricopeptide repeats 1 ELISA Kit |
| SKU: | HUFI01406 |
| Reactivity: | Human |
| Assay Type: | Sandwich ELISA, Double Antibody |
| Sensitivity: | 0.094 ng/mL |
| Range: | 0.156-10 ng/mL |
| Sample Type: | Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate, Other Liquid Samples |
| Storage: | 2-8°C for 12 months. |
| Linearity: |
| ||||||||||||||||
| Recovery: |
|
Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.
| Step | Procedure |
| 1 | Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions. |
| 2 | Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding. |
| 3 | Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes. |
| 4 | HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes. |
| 5 | Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes. |
| 6 | Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately. |
| Sample Type | Protocol |
| Serum | Allow blood to clot, centrifuge at 1000 × g for 20 minutes, collect supernatant supernatant and store appropriately. |
| Plasma | Collect using anticoagulant tubes, centrifuge at 1000 × g for 15 minutes at 2–8°C and collect plasma. |
| Tissue Homogenate | Homogenize tissue in PBS with protease inhibitors, centrifuge and collect supernatant. |
| Cell Culture Supernatant | Centrifuge at 2500 rpm for 5 minutes and collect clarified supernatant. |
| Cell Lysate | Lyse cells using lysis buffer with protease inhibitors, centrifuge and collect protein supernatant. |
| Other Sample Types | For more information about how to process other sample types, (e.g., body fluids, breast milk & more), please contact our Tech Support Team at techsupport@assaygenie.com. |
| Component | Quantity | Storage | |
| 48T | 96T | ||
| ELISA Microplate (Dismountable) | 8×6 | 8×12 | Place the test strips into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Lyophilized Standard | 1 vial | 2 vial | Place the standards into a sealed foil bag with the desiccant. Store for 1 month at 2-8°C; Store for 12 months at -20°C. |
| Biotin-labeled Antibody (Concentrated, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| HRP-Streptavidin Conjugate (SABC, 100X) | 60 ul | 120 ul | 2-8°C (Avoid direct light) |
| TMB Substrate | 5 ml | 10 ml | 2-8°C (Avoid direct light) |
| Sample Dilution Buffer | 10 ml | 20 ml | 2-8°C |
| Antibody Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| SABC Dilution Buffer | 5 ml | 10 ml | 2-8°C |
| Stop Solution | 5 ml | 10 ml | 2-8°C |
| Wash Buffer(25X) | 15 ml | 30 ml | 2-8°C |
| Plate Sealer | 3 pieces | 5 pieces | - |
| Technical Manual | 1 copy | 1 copy | - |
Related Products
Human Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) ELISA Kit (HUEB1641)
Human Immunology ELISA Kits 8
Mouse Interferon-induced protein with tetratricopeptide repeats 1 (Ifit1) ELISA Kit (MOEB1397)
Mouse Cell Signalling ELISA Kits 4
Human IFITM1 (Interferon-induced transmembrane protein 1) ELISA Kit (HUFI07774)
Human Interferon-induced protein 44 (IFI44) ELISA Kit (HUEB2447)
Human Immunology ELISA Kits 10
Human Interferon-inducible protein AIM2 (AIM2) ELISA Kit (HUEB2205)
Human Immunology ELISA Kits 9