The Human GREM1 (Gremlin-1) ELISA Kit is specifically designed for the precise measurement of Gremlin-1 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results, perfect for various research purposes.Gremlin-1 is a vital protein implicated in inhibiting bone morphogenetic protein (BMP) signaling and has been linked to various diseases, including cancer, fibrosis, and developmental disorders. Studying Gremlin-1 levels can provide valuable insights into disease mechanisms and potential therapeutic targets, making this ELISA kit a valuable tool for researchers in the field.
Product Name:
Human GREM1 (Gremlin 1) ELISA Kit
SKU:
HUES02396
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
0.1 ng/mL
Detection range:
0.16-10 ng/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
