Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit
The Human GM-CSF (Granulocyte-Macrophage Colony-Stimulating Factor) ELISA Kit is a highly sensitive and specific assay designed for the accurate quantification of GM-CSF levels in human serum, plasma, and cell culture supernatants. This kit is a valuable tool for researchers studying the role of GM-CSF in various physiological and pathological processes.GM-CSF is a cytokine that plays a crucial role in the regulation of immune responses, hematopoiesis, and inflammation. Aberrant GM-CSF levels have been implicated in autoimmune diseases, cancer, and other inflammatory conditions, making it a key target for therapeutic interventions. With its high performance and ease of use, the Human GM-CSF ELISA Kit from AssayGenie provides researchers with a reliable and reproducible method for measuring GM-CSF levels in biological samples. Whether investigating disease mechanisms or evaluating potential therapies, this kit offers researchers a valuable tool for advancing their research.
Product Name:
Human GM-CSF (Granulocyte Macrophage Colony Stimulating Factor) ELISA Kit
SKU:
HUES01368
Size:
96 Assays
Detection Method:
Colorimetric method, ELISA, Sandwich
Assay type:
Sandwich-ELISA
Assay time:
3 h 30 min
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Reovery:
80%-120%
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
