Human GM-CSF/CSF2 Recombinant Protein (E.coli) (RPES2386)
- SKU:
- RPES2386
- Product Type:
- Recombinant Protein
- Species:
- Human
Description
Human GM-CSF/CSF2 Recombinant Protein (E.coli)
Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially identified as a growth factor that can aid in the cell proliferation of granulocyte-macrophage progenitors in culture. It is made by a variety of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts) in response to immune and inflammatory cytokines. GM-CSF is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF promotes endothelial cell migration and proliferation in human cells. Additionally, GM-CSF has been found to promote the proliferation of osteogenic sarcoma; carcinoma and adenocarcinoma cell lines in culture. Human GM-CSF/CSF2 Recombinant Protein (E.coli) is a highly pure recombinant protein developed by Assay Genie for use in a range of applications.
| Product Name: | Human GM-CSF/CSF2 Recombinant Protein (E.coli) (RPES2386) |
| Product Code: | RPES2386 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Granulocyte-Macrophage Colony-Stimulating Factor, GM-CSF, Colony-Stimulating Factor, CSF, Molgramostin, Sargramostim, CSF2, GMCSF |
| Accession: | P04141 |
| Sequence: | Ala18-Glu144 |
| Activity: | Measured in a cell proliferation assay using TF?1 human erythroleukemic cells. The ED50 for this effect is 4-20 pg/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Granulocyte-Macrophage Colony-Stimulating Factor is produced by our E.coli expression system and the target gene encoding Ala18-Glu144 is expressed. |
| Purity: | > 95% as determined by reducing SDS-PAGE. |
| Mol Mass: | 14.6 kDa |
| AP Mol Mass: | 14 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of PBS; pH 7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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