Human Immunology ELISA Kits 12
Human FSH (Follicle-Stimulating Hormone) ELISA Kit (HUES02229)
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- SKU:
- HUES02229
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P01225(FSH beta)
- ELISA Type:
- Sandwich
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Assay Type:
- Sandwich-ELISA
- Sensitivity:
- 0.94 mIU/mL
- Range:
- 1.56-100 mIU/mL
- Recovery:
- 80%-120%
Frequently bought together:
Description
Human FSH (Follicle-Stimulating Hormone) ELISA Kit
The Human FSH (Follicle Stimulating Hormone) ELISA Kit is a specialized assay designed for the accurate measurement of FSH levels in human samples, including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results, making it well-suited for a variety of research applications.FSH is a key hormone involved in the regulation of reproductive processes, specifically in the development and maturation of ovarian follicles in women and sperm production in men. Abnormal FSH levels can indicate issues with fertility, hormonal imbalances, or other reproductive disorders, making it an important biomarker for studying these conditions and developing targeted treatment strategies.By utilizing the Human FSH ELISA Kit, researchers can accurately measure FSH levels in samples, leading to a better understanding of reproductive health and potential therapies for related conditions. This kit provides a reliable and efficient tool for studying FSH levels in various research settings.
| Product Name: | Human FSH (Follicle-Stimulating Hormone) ELISA Kit |
| SKU: | HUES02229 |
| Size: | 96 Assays |
| Detection Method: | Colorimetric method, ELISA, Sandwich |
| Assay type: | Sandwich-ELISA |
| Assay time: | 3 h 30 min |
| Sensitivity: | 0.94 mIU/mL |
| Detection range: | 1.56-100 mIU/mL |
| Reovery: | 80%-120% |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
| Component | Specification | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T | -20°C, 12 months |
| Reference Standard | 96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials | -20°C, 12 months |
| Concentrated Biotinylated Detection Ab(100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C, 12 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C (Protect from light), 12 months |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL | 2–8°C, 12 months |
| Biotinylated Detection Ab Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| HRP Conjugate Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL | 2–8°C, 12 months |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C |
| Plate Sealer | 96T/48T/24T: 5 pieces | 96T*5: 25 pieces | 2–8°C |
| Technical Manual | 1 copy | - |
| Certificate of Analysis | 1 copy | - |
| Linearity: |
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| Recovery: |
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| Precision: |
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| Uniport ID: | P01225 |
| Sample type & Sample volume: | Serum, Plasma And Other Biological Fluids; 100 μL |
| Specificity: | This kit recognizes Human FSH in samples. No significant cross-reactivity or interference between Human FSH and analogues was observed |
| Storage: | 2-8℃ |
| Research Area: | Cancer,Metabolism,Signal Transduction |
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