Human Immunology ELISA Kits 12
Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit (HUES01362)
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- SKU:
- HUES01362
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P49771
- ELISA Type:
- Sandwich
- Synonyms:
- FLT3LG, Flt3-L, FL, Fms-Related Tyrosine Kinase 3 ligand
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Assay Type:
- Sandwich-ELISA
- Sensitivity:
- 9.38 pg/mL
- Range:
- 15.63-1000 pg/mL
- Recovery:
- 80%-120%
Frequently bought together:
Description
Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit
The Human FLT3L (FMS-like tyrosine kinase 3 Ligand) ELISA Kit is a reliable tool for measuring FLT3L levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it suitable for a variety of research applications.FLT3L is a key cytokine involved in the regulation of hematopoiesis and immune responses. It plays a crucial role in the development of dendritic cells and influences the differentiation of various immune cell types. Dysregulation of FLT3L has been associated with conditions such as leukemia and autoimmune diseases, making it a valuable biomarker for studying these disorders and developing targeted therapies.Overall, the Human FLT3L ELISA Kit is essential for researchers interested in investigating the role of FLT3L in health and disease, providing a reliable method for quantifying FLT3L levels in biological samples.
| Product Name: | Human Flt3L (FMS Like Tyrosine Kinase 3 Ligand) ELISA Kit |
| SKU: | HUES01362 |
| Size: | 96 Assays |
| Detection Method: | Colorimetric method, ELISA, Sandwich |
| Assay type: | Sandwich-ELISA |
| Assay time: | 3 h 30 min |
| Sensitivity: | 9.38 pg/mL |
| Detection range: | 15.63-1000 pg/mL |
| Reovery: | 80%-120% |
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to the target protein. Standards or samples are added to the micro ELISA plate wells and bind to the immobilized antibody. A biotinylated detection antibody specific to the target protein is then added, followed by Avidin-Horseradish Peroxidase (HRP) conjugate. Free components are washed away. The substrate solution is added to each well, resulting in a color change. Only wells containing the target protein, detection antibody, and HRP conjugate will develop a blue color. The reaction is terminated by the addition of stop solution, resulting in a yellow color. The optical density (OD) is measured at 450 nm ± 2 nm. The OD value is directly proportional to the concentration of the target protein in the sample and is determined using a standard curve.
| Component | Specification | Storage |
| Micro ELISA Plate (Dismountable) | 96T: 8 wells × 12 strips | 48T: 8 wells × 6 strips | 24T: 8 wells × 3 strips | 96T*5: 5 plates, 96T | -20°C, 12 months |
| Reference Standard | 96T: 2 vials | 48T/24T: 1 vial | 96T*5: 10 vials | -20°C, 12 months |
| Concentrated Biotinylated Detection Ab(100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C, 12 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL | 48T/24T: 1 vial, 60 μL | 96T*5: 5 vials, 120 μL | -20°C (Protect from light), 12 months |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL | 96T*5: 5 vials, 20 mL | 2–8°C, 12 months |
| Biotinylated Detection Ab Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| HRP Conjugate Diluent | 96T/48T/24T: 1 vial, 14 mL | 96T*5: 5 vials, 14 mL | 2–8°C, 12 months |
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL | 96T*5: 5 vials, 30 mL | 2–8°C, 12 months |
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C (Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL | 96T*5: 5 vials, 10 mL | 2–8°C |
| Plate Sealer | 96T/48T/24T: 5 pieces | 96T*5: 25 pieces | 2–8°C |
| Technical Manual | 1 copy | - |
| Certificate of Analysis | 1 copy | - |
| Linearity: |
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| Recovery: |
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| Precision: |
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| Uniport ID: | P49771 |
| Sample type & Sample volume: | Serum, Plasma And Other Biological Fluids; 100 μL |
| Specificity: | This kit recognizes Human Flt3L in samples. No significant cross-reactivity or interference between Human Flt3L and analogues was observed |
| Storage: | 2-8℃ |
| Research Area: | Cancer,Cardiovascular,Immunology |
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