Human Fibromodulin/FMOD ELISA Kit (HUFI01391)

Human Fibromodulin/FMOD ELISA Kit

The Human Fibromodulin (FMOD) ELISA Kit is a highly sensitive and specific assay designed for the precise detection of fibromodulin levels in human serum, plasma, and cell culture supernatants. Fibromodulin is a key extracellular matrix protein that plays a crucial role in tissue development and remodeling. This kit offers reliable and reproducible results, making it an ideal tool for researchers studying fibromodulin's involvement in various physiological and pathological processes.

Fibromodulin has been implicated in conditions such as wound healing, arthritis, and cancer, highlighting its potential as a biomarker for disease progression and therapeutic targeting. With its high accuracy and ease of use, the Human Fibromodulin ELISA Kit is a valuable asset for researchers looking to further understand the role of fibromodulin in health and disease.

Note:The below protocol is a sample protocol. Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Step	Procedure
1	Reagent & Plate Preparation: Equilibrate reagents and TMB substrate to room temperature. Set standard, test sample and control (zero) wells on the pre-coated plate and record their positions.
2	Primary Incubation: Prepare standards, samples, blanks and load into designated wells. Incubate plate at 37°C for 90 minutes to allow antigen binding.
3	Detection Antibody Binding: Add biotin-labeled detection antibody and incubate at 37°C for 60 minutes.
4	HRP-Streptavidin Binding: Add HRP-Streptavidin (SABC) and incubate at 37°C for 30 minutes.
5	Color Development: Add TMB substrate and incubate in the dark for 10–20 minutes.
6	Stop Reaction & Reading: Add stop solution and measure absorbance at 450 nm immediately.